Eric J. Stockinger
Department of Horticulture and Crop Science
The Ohio State University/OARDC
Wooster OH 44691
stockinger.4@osu.edu
http://www.oardc.ohio-state.edu/stockingerlab/
| University of California, Riverside | Plant Science | B.S. | 1985 |
| University of California, Riverside | Botany (Plant Genetics) | Ph.D. | 1993 |
| Michigan State University, East Lansing | Plant Genetics | Postdoc | 1993-1994 |
| Michigan State University, East Lansing | Gene Regulation | Postdoc | 1994-2000 |
Appointments
| 2006-Present | Associate Professor | Dept of Horticulture and Crop Science. The Ohio State University/OARDC, Wooster OH 44691 |
| 2000-2006 | Assistant Professor | Dept of Horticulture and Crop Science. The Ohio State University/OARDC, Wooster OH 44691 |
| 1994-2000 | Postdoctoral Research Assistant | Dept of Crop & Soil Sciences, Michigan State University, East Lansing MI Advisor: Michael F. Thomashow |
| 1993-1994 | Postdoctoral Research Assistant | Dept of Horticulture, Michigan State University, East Lansing MI Advisor: Amy F. Iezzoni |
| 1986-1993 | Research Assistant | Dept of Botany & Plant Sciences, University of California Riverside CA Advisor: Linda L. Walling |
| 1990 | Teaching Assistant (Molecular Biology) | Dept of Biology, University of California, Riverside CA Advisor: Brad Hyman |
| 1986 | Teaching Assistant (Genetics) | Dept of Biology, University of California, Riverside CA Advisor: Gary Jones |
| 1984-1986 | Undergraduate Research Assistant | Dept of Botany & Plant Sciences, University of California, Riverside CA Advisor: J. Giles Waines |
Research interests and current focus
Mechanistic understanding of freezing tolerance and winter hardiness in the Triticeae cereals at the gene and gene regulatory level.
Development of winter malting barley adapted to Ohio and the neighboring Great Lake and Midwest state regions.
2017–2022 Elected Representative
National Barley Improvement Committee North Central Region
Professional Service
2013–present. OSU-OARDC Crop Variety Release and Distribution Committee
Courses taught
Agricultural genomics (https://hcs.osu.edu/courses/hcs-700302)
Plant Breeding and Biotechnology (https://hcs.osu.edu/courses/hcs-7625)
Yeast one-hybrid (laboratory exercise)
Publications
Peer-reviewed publications
Stockinger, E. J. (2021). The breeding of winter-hardy malting barley. Plants (Basel) 10 (7). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309344/
Munoz-Amatriain, M., Hernandez, J., Herb, D., Baenziger, P. S., Bochard, A.M., Capettini, F., Casas, A., Cuesta-Marcos, A., Einfeldt, C., Fisk, S., Genty, A., Helgerson, L., Herz, M., Hu, G., Igartua, E., Karsai, I., Nakamura, T., Sato, K., Smith, K., Stockinger, E., Thomas, W., Hayes, P. (2020). Perspectives on low temperature tolerance and vernalization sensitivity in barley: prospects for facultative growth habit. Front. Plant Sci. 11 (1625). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814503/
Kost, M.A., Perales, H., Wijeratne, S., Wijeratne, A.J., Stockinger, E.J., and Mercer, K.L. (2020). Transcriptional differentiation of UV-B protectant genes in maize landraces spanning an elevational gradient in Chiapas, Mexico. Evol. Appl. 13: 1949-1967. https://www.ncbi.nlm.nih.gov/pubmed/32908597
Neyhart, J.L., Sweeney, D., Sorrells, M., Kapp, C., Kephart, K.D., Sherman, J., Stockinger, E.J., Fisk, S., Hayes, P., Daba, S., Mohammadi, M., Hughes, N., Lukens, L., González Barrios, P., Gutiérrez, L., and Smith, K.P. (2019) Registration of the S2MET Barley Mapping Population for Multi-Environment Genomewide Selection. J. Plant Reg. 13:270-280. https://acsess.onlinelibrary.wiley.com/doi/abs/10.3198/jpr2018.06.0037crmp
Dhillon, T., Morohashi, K., and Stockinger, E.J. (2017) CBF2A-CBF4B genomic region copy numbers alongside the circadian clock play key regulatory mechanisms driving expression of FR-H2 CBFs. Plant Mol. Biol. 94: 333-347. https://www.ncbi.nlm.nih.gov/pubmed/28434151
Kost, M.A., Perales, H.R., Wijeratne, S., Wijeratne, A.J., Stockinger, E., Mercer, K.L. (2017) Differentiated transcriptional signatures in the maize landraces of Chiapas, Mexico. BMC Genomics 18: 707. https://www.ncbi.nlm.nih.gov/pubmed/28886704
Wang, Y.J., Ballard, H.E., Sternberger, A.L., Wyatt, S.E., Stockinger, E.J., and Nadella, V. (2017) The potential role of two LEAFY orthologs in the chasmogamous/cleistogamous mixed breeding system of Viola pubescens (Violaceae). J. Torrey Botan. Society 144: 206-217. http://www.bioone.org/doi/full/10.3159/TORREY-D-16-00023R2
Jeknić, Z., Pillman, K.A., Dhillon, T., Skinner, J.S., Veisz, O., Cuesta-Marcos, A., Hayes, P.M., Jacobs, A.K., Chen, T.H.H., and Stockinger, E.J. (2014). Hv-CBF2A overexpression in barley accelerates COR gene transcript accumulation and acquisition of freezing tolerance during cold acclimation. Plant Mol. Biol. 84: 67-82. http://www.ncbi.nlm.nih.gov/pubmed/23949371
Dhillon, T., and Stockinger, E.J. (2013). Cbf14 copy number variation in the A, B, and D genomes of diploid and polyploid wheat. Theor. Appl. Genet. 126, 2777-2789. http://www.ncbi.nlm.nih.gov/pubmed/23918064
Li, C., Rudi, H., Stockinger, E.J., Cheng, H., Cao, M., Fox, S.E., Mockler, T.C., Westereng, B., Fjellheim, S., Rognli, O.A., and Sandve, S.R. (2012). Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses. BMC Plant Biol. 12: 65. http://www.ncbi.nlm.nih.gov/pubmed/22569006
Dhillon, T., Pearce, S.P., Stockinger, E.J., Distelfeld, A., Li, C., Knox, A.K., Vashegyi, I., Vágújfalvi, A., Galiba, G., and Dubcovsky, J. (2010) Freezing tolerance and flowering regulation in cereals: the VRN-1 connection. Plant Physiol. 153: 1846-1858. http://www.ncbi.nlm.nih.gov/pubmed/20571115
Knox A.K., Dhillon, T., Cheng, H., Tondelli, A., Pecchioni, N., and Stockinger, E.J. (2010) CBF gene copy number variation at Frost Resistance-2 is associated with levels of freezing tolerance in temperate-climate cereals. Theor. Appl. Genet. 121: 21-35. http://www.ncbi.nlm.nih.gov/pubmed/20213518
Dominguez, T., Hernandez, M.L., Pennycooke, J.C., Jimenez, P., Martinez-Rivas, J.M., Sanz, C., Stockinger, E.J., Sanchez-Serrano, J.J., and Sanmartin, M. (2010) Increasing ω-3 desaturase expression in tomato results in altered aroma profile and enhanced resistance to cold stress. Plant Physiol. 153: 655-665. http://www.ncbi.nlm.nih.gov/pubmed/20382895
Pennycooke, J.C., Cheng, H., Roberts, S.M., Yang, Q., Rhee, S.Y., and Stockinger, E.J. (2008) The low temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a genomic environment undergoing gene duplications, deletions, and rearrangements. Plant Mol. Biol. 67, 483-497. http://www.ncbi.nlm.nih.gov/pubmed/18415686
Pennycooke, J.C., Cheng, H. and Stockinger, E.J. (2008) Comparative genomic sequence and expression analyses of Medicago truncatula and alfalfa subspecies falcata COLD-ACCLIMATION-SPECIFIC genes. Plant Physiol. 146, 1242-1254. http://www.ncbi.nlm.nih.gov/pubmed/18218976
Knox, A.K., Li, C., Vagujfalvi, A., Galiba, G., Stockinger, E.J., and Dubcovsky, J. (2008) Identification of candidate CBF genes for the frost tolerance locus Fr-Am2 in Triticum monococcum. Plant Mol Biol. 67, 257-270. http://www.ncbi.nlm.nih.gov/pubmed/18317935
Xiao, H., Jiang, N., Schaffner, E., Stockinger, E.J., and van der Knaap, E. (2008) A retrotransposon-mediated gene duplication underlies morphological variation of tomato fruit. Science 319, 1527-1530. http://www.ncbi.nlm.nih.gov/pubmed/18339939
Stockinger, E.J., Skinner, J.S., Gardner, K.G., Francia, E., and Pecchioni, N. (2007) Expression levels of barley Cbf genes at the Frost resistance-H2 locus are dependent upon alleles at Fr-H1 and Fr-H2. Plant J. 51:308-321. http://www.ncbi.nlm.nih.gov/pubmed/17559507
Francia, E., Barabaschi, D., Tondelli, A., Laidò, G., Rizza, F., Stanca, A.M. Busconi, M., Fogher, C., Stockinger, E.J., and Pecchioni, N. (2007) Fine mapping of a HvCBF gene cluster at the frost resistance locus Fr-H2 in barley. Theor. Appl. Genet. 115:1083-1091. http://www.ncbi.nlm.nih.gov/pubmed/17763839
Tondelli, A., Francia, E., Barabaschi, D., Aprile, A., Skinner, J.S., Stockinger, E.J., Stanca, A.M., and Pecchioni, N. (2006) Mapping regulatory genes as candidates for cold and drought stress tolerance in barley. Theor. Appl. Genet. 112:445-454. http://www.ncbi.nlm.nih.gov/pubmed/16315028
Skinner, J.S., Szûcs, P., von Zitzewitz, J., Marquez-Cedillo, L., Filichkin, T., Stockinger, E.J., Chen, T.H.H., and Hayes P.M. (2006) Mapping of barley homologs to genes that regulate low temperature tolerance in Arabidopsis. Theor. Appl. Genet. 112:832-842. http://www.ncbi.nlm.nih.gov/pubmed/16365758
Skinner, J.S., von Zitzewitz, J., Szûcs, P., Marquez-Cedillo, L., Filichkin, T., Amundsen, K., Stockinger, E.J., Thomashow, M.F., Chen, T.H.H., and Hayes P.M. (2005) Structural, functional, and phylogenetic characterization of a large CBF gene family in barley. Plant Mol. Biol. 59:533-551. http://www.ncbi.nlm.nih.gov/pubmed/16244905
Wang, Z., Triezenberg, S.J., Thomashow, M.F., and Stockinger E.J. (2005) Multiple hydrophobic motifs in Arabidopsis CBF1 COOH-terminus provide functional redundancy in trans-activation. Plant Mol. Biol. 58:543-559. http://www.ncbi.nlm.nih.gov/pubmed/16021338
Zhang, X., Fowler, S.G., Cheng, H., Lou, Y., Rhee, S.Y., Stockinger, E.J., and Thomashow, M.F. (2004) Freezing sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing tolerant Arabidopsis. Plant J. 39:905-919. http://www.ncbi.nlm.nih.gov/pubmed/15341633
Coaker, G.L., Willard, B., Kinter, M., Stockinger, E.J., and Francis, D.M. (2004) Proteomic analysis of resistance mediated by Rcm 2.0 and Rcm 5.1, two loci controlling resistance to bacterial canker of tomato. Mol. Plant Microbe Interact. 17:1019-1028. http://www.ncbi.nlm.nih.gov/pubmed/15384492
Stockinger, E.J., Mao, Y., Regier, M., Triezenberg, S.J. and Thomashow, M.F. (2001) Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Nucleic Acids Res. 29:1524-1533. http://www.ncbi.nlm.nih.gov/pubmed/11266554
Thomashow, M.F., Gilmour, S.J., Stockinger, E.J., Jaglo-Ottosen, K.R., Zarka, D.G. (2001) Role of the Arabidopsis CBF transcriptional activators in cold acclimation. Physiol. Plant. 112:171-175.
Gilmour, S.J., Zarka, D.G., Stockinger, E.J., Salazar, M.P., Houghton, J.M., and Thomashow, M.F. (1998) Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J. 16:433-442. http://www.ncbi.nlm.nih.gov/pubmed/9881163
Stockinger, E.J., Gilmour, S.J. and Thomashow, M.F. (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad. Sci. U.S.A. 94:1035-1040. http://www.ncbi.nlm.nih.gov/pubmed/9023378
Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., Gilmour, S.J. and Zarka, D.G. (1997) Function and regulation of Arabidopsis thaliana COR (cold-regulated) genes. ACTA Physiol. Planta. 19:497-504. http://www.springerlink.com/content/8528824533846tu4/
Garvin, D.F., Federici, C.V., Stockinger, E.J., and Waines, J.G. (1997) Genetic marker transmission in early generation common x tepary bean hybrids. J. Hered. 88:537-540. http://jhered.oxfordjournals.org/content/88/6/537
Stockinger, E.J., Mulinix, C.A., Long, C.M., Brettin, T.S. and Iezzoni, A.F. (1996) A linkage map of sweet cherry based on RAPD analysis of a microspore-derived callus culture population. J. Hered. 87:214-218. http://www.ncbi.nlm.nih.gov/pubmed/8683097
Stockinger, E.J., and Walling, L.L. (1994) Pto3 and Pto4: novel genes from Lycopersicon hirsutum var. glabratum conferring resistance to Pseudomonas syringae pv. tomato. Theor. Appl. Genet. 89:879-884. http://www.springerlink.com/content/l64qx21741w46496/
Stockinger, E.J., and Walling, L.L. (1994) A chlorophyll a/b-binding protein gene from soybean (Glycine max. [L.] Merr). Plant Physiol. 104:1475-1476. http://www.ncbi.nlm.nih.gov/pubmed/8016279
Demmin, D.S., Stockinger, E.J., Chang, Y.C., and Walling, L.L. (1989) Phylogenetic relationships between chlorophyll a/b-binding protein (CAB) multigene family: An intra - and interspecies study. J. Mol. Evol. 29:266-279. http://www.ncbi.nlm.nih.gov/pubmed/2506360
Non-refereed technical publications of relevance
Culman, S., Lindsey, L., Loux, M., Paul, P., and Stockinger, E. (2018) Management of Ohio winter malting barley. Ohio State University Extension. https://stepupsoy.osu.edu/sites/hcs-soy/files/barley%20guide.pdf
Book Chapters
Galiba, G., Stockinger, E.J., Francia, E., Milc, J., Kocsy, G., and Pecchioni, N. (2013). Freezing tolerance in the Triticeae. In Translational Genomics for Crop Breeding, R.K. Varshney, and R. Tuberosa, eds. (USA: Wiley), pp. 99–124.
Stockinger, E.J. (2011). Pipkin. In, “The Oxford Companion to Beer,” G. Oliver (ed.) Oxford University Press, New York. pp. 656.
Stockinger E.J. (2009) Winter hardiness and the CBF genes in the Triticeae. In, “Plant Cold Hardiness: From the Laboratory to the Field,” LV Gusta, M Wisniewski and KK Tanino, (eds.), CABI Publishing Oxon, UK. pp. 119-130.
Stockinger, E.J., Cheng, H., and Skinner, J.S. (2005) Structural organization of barley CBF genes coincident with a QTL for cold hardiness. In, “Cold Hardiness in Plants,” THH Chen, M Uemura and S Fujikawa, (eds.), CABI Publishing, Wallingford, Oxon, UK. pp. 53-63.
Skinner, J.S., von Zitzewitz, J., Marquez-Cedillo, L., Filichkin, T., Szûcs, P., Amundsen, K., Stockinger E.J., Thomashow, M.F., Chen, T.H.H., and Hayes, P.M. (2006) Barley Contains a large CBF gene family associated with quantitative cold tolerance traits. In, “Cold Hardiness in Plants,” THH Chen, M Uemura and S Fujikawa, (eds.), CABI Publishing Oxon, UK pp. 30-52.
Thomashow, M.F., Stockinger, E.J., and Gilmour, S.J. (1997) Regulation of plant gene expression in response to low temperature. In, “Plant Cold Hardiness. Molecular Biology, Biochemistry and Physiology,” PH Li and THH Chen (eds.), Plenum, New York. pp. 29-34.
Thomashow, M.F., Artus, N.N., Bloksberg, L.N., Gilmour, S.J., Stockinger, E.J., Wilhelm, K., Uemura, M., Webb, M.S., and Steponkus, P.L. (1996) Cold acclimation in Arabidopsis thaliana: function and regulation of COR genes. In, “Physical Stresses in Plants. Genes and their Products for Tolerance,” S. Grillo and A. Leone (eds.) Springer, Berlin. pp. 71-81.
Patents Awarded
Thomashow, M.F., Gilmour, S.J., Jiang, C-Z., Fromm, M., Zarka, D.G., Jaglo-Ottosen, K.R., Stockinger, E.J., Zhang, J., and Haake, V. (2004) Plant having altered environmental stress tolerance. Patent no. 6,706,866
Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., Gilmour, S.J., Zarka, D.G., and Jiang, C-Z. (2002) Plant having altered environmental stress tolerance. Patent no. 6,417,428
Thomashow, M.F., and Stockinger, E.J. (1999) DNA and encoded protein which regulates cold and dehydration regulated genes. Patent no. 5,965,705
Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., and Zarka, D.G. (1999) Plant material containing non-naturally introduced binding protein for regulating cold and dehydration regulatory genes. Patent no. 5,929,305
Thomashow, M.F., and Stockinger, E.J. (1999) DNA and encoded protein which regulates cold and dehydration regulated genes. Patent no. 5,892,009
Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., Zarka, D.G., and Gilmour, S.J. (1999) Method for regulating cold and dehydration regulatory genes in a plant. Patent no. 5,891,859