Eric J. Stockinger

 

Eric Stockinger

Eric J. Stockinger

Department of Horticulture and Crop Science

The Ohio State University/OARDC

Wooster OH 44691

stockinger.4@osu.edu

http://www.stockingerlab.osu.edu

 

(Photo Credit to Karen Skubik of The Wooster Weekly News)

Professional Preparation

University of California, Riverside CA            Plant Science                           B.S.      1985

University of California, Riverside CA            Botany (Plant Genetics)     Ph.D.         1993

Michigan State University, East Lansing MI     Plant Genetics                    Postdoc      1993-1994

Michigan State University, East Lansing MI     Gene Regulation                Postdoc      1994-2000

 

Appointments

2006-Present Associate Professor, Dept of Horticulture and Crop Science. The Ohio State University/OARDC, Wooster OH 44691

2000-2006    Assistant Professor, Dept of Horticulture and Crop Science. The Ohio State University/OARDC, Wooster OH 44691

1994-2000    Postdoctoral Research Assistant, Dept of Crop & Soil Sciences, Michigan State University, East Lansing MI; Research Advisor: Michael F. Thomashow

1993-1994    Postdoctoral Research Assistant, Dept of Horticulture, Michigan State University, East Lansing MI; Research Advisor: Amy F. Iezzoni

1986-1993    Research Assistant, Dept of Botany & Plant Sciences, University of California Riverside CA; Research Advisor: Linda L. Walling

1990             Teaching Assistant (Molecular Biology), Dept of Biology, University of California, Riverside CA; Teaching Advisor: Brad Hyman

1986             Teaching Assistant (Genetics), Dept of Biology, University of California, Riverside CA; Teaching Advisor: Gary Jones

1984-1986    Undergraduate Research Assistant, Dept of Botany & Plant Sciences, University of California, Riverside CA; Research Advisor: J. Giles Waines

 

Research interests and current focus

Mechanistic understanding of freezing tolerance and winter hardiness in the Triticeae cereals at the gene and gene regulatory level.

Development of winter malting barley adapted to Ohio and the neighboring Great Lake and Midwest state regions.

 

Courses taught

Agricultural genomics (http://plantpath.osu.edu/plntpth-7003, co-instructor)

Yeast One-Hybrid (laboratory exercise)

Molecular Genetic Control of Flowering: Integration of Environmental Signals with the Endogenous Program of Plant Development (journal club)

Transcription factors controlling plant traits of agroeconomic value (journal club)

Crop Breeding (guest lecture, plant cellular organization and genetic structure)

 

Publications

Dhillon, T., Morohashi, K., and Stockinger, E.J. (2017) CBF2A-CBF4B genomic region copy numbers alongside the circadian clock play key regulatory mechanisms driving expression of FR-H2 CBFs. Plant Mol. Biol. 94: 333-347. https://www.ncbi.nlm.nih.gov/pubmed/28434151

Jeknić, Z., Pillman, K.A., Dhillon, T., Skinner, J.S., Veisz, O., Cuesta-Marcos, A., Hayes, P.M., Jacobs, A.K., Chen, T.H.H., and Stockinger, E.J. (2014). Hv-CBF2A overexpression in barley accelerates COR gene transcript accumulation and acquisition of freezing tolerance during cold acclimation. Plant Mol. Biol. 84: 67-82. http://www.ncbi.nlm.nih.gov/pubmed/23949371

Dhillon, T., and Stockinger, E.J. (2013). Cbf14 copy number variation in the A, B, and D genomes of diploid and polyploid wheat. Theor. Appl. Genet. 126, 2777-2789. http://www.ncbi.nlm.nih.gov/pubmed/23918064

Li, C., Rudi, H., Stockinger, E.J., Cheng, H., Cao, M., Fox, S.E., Mockler, T.C., Westereng, B., Fjellheim, S., Rognli, O.A., and Sandve, S.R. (2012). Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses. BMC Plant Biol. 12: 65. http://www.ncbi.nlm.nih.gov/pubmed/22569006

Dhillon, T., Pearce, S. P., Stockinger, E. J., Distelfeld, A., Li, C., Knox, A. K., Vashegyi, I., Vágújfalvi, A., Galiba, G., and Dubcovsky, J. (2010) Freezing tolerance and flowering regulation in cereals: the VRN-1 connection. Plant Physiol. 153: 1846-1858. http://www.ncbi.nlm.nih.gov/pubmed/20571115

Knox A.K., Dhillon, T., Cheng, H., Tondelli, A., Pecchioni, N., and Stockinger, E.J. (2010) CBF gene copy number variation at Frost Resistance-2 is associated with levels of freezing tolerance in temperate-climate cereals. Theor. Appl. Genet. 121: 21-35. http://www.ncbi.nlm.nih.gov/pubmed/20213518

Dominguez, T., Hernandez, M.L., Pennycooke, J.C., Jimenez, P., Martinez-Rivas, J.M., Sanz, C., Stockinger, E.J., Sanchez-Serrano, J.J., and Sanmartin, M. (2010) Increasing ω-3 desaturase expression in tomato results in altered aroma profile and enhanced resistance to cold stress. Plant Physiol. 153: 655-665. http://www.ncbi.nlm.nih.gov/pubmed/20382895

Pennycooke, J.C., Cheng, H., Roberts, S.M., Yang, Q., Rhee, S.Y., and Stockinger, E.J. (2008) The low temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a genomic environment undergoing gene duplications, deletions, and rearrangements. Plant Mol. Biol. 67, 483-497. http://www.ncbi.nlm.nih.gov/pubmed/18415686

Pennycooke, J.C., Cheng, H. and Stockinger, E.J. (2008) Comparative genomic sequence and expression analyses of Medicago truncatula and alfalfa subspecies falcata COLD-ACCLIMATION-SPECIFIC genes. Plant Physiol. 146, 1242-1254. http://www.ncbi.nlm.nih.gov/pubmed/18218976

Knox, A.K., Li, C., Vagujfalvi, A., Galiba, G., Stockinger, E.J., and Dubcovsky, J. (2008) Identification of candidate CBF genes for the frost tolerance locus Fr-Am2 in Triticum monococcum. Plant Mol Biol. 67, 257-270. http://www.ncbi.nlm.nih.gov/pubmed/18317935

Xiao, H., Jiang, N., Schaffner, E., Stockinger, E.J., and van der Knaap, E. (2008) A retrotransposon-mediated gene duplication underlies morphological variation of tomato fruit. Science 319, 1527-1530. http://www.ncbi.nlm.nih.gov/pubmed/18339939

Stockinger, E.J., Skinner, J.S., Gardner, K.G., Francia, E., and Pecchioni, N. (2007) Expression levels of barley Cbf genes at Frost resistanceH2 are dependent upon alleles at Fr‑H1 and Fr‑H2. Plant J. 51:308-321. http://www.ncbi.nlm.nih.gov/pubmed/17559507

Francia, E., Barabaschi, D., Tondelli, A., Laidò, G., Rizza, F., Stanca, A.M. Busconi, M., Fogher, C., Stockinger, E.J., and Pecchioni, N. (2007) Fine mapping of a HvCBF gene cluster at the frost resistance locus Fr-H2 in barley. Theor. Appl. Genet. 115:1083-1091. http://www.ncbi.nlm.nih.gov/pubmed/17763839

Tondelli, A., Francia, E., Barabaschi, D., Aprile, A., Skinner, J.S., Stockinger, E.J., Stanca, A.M., and Pecchioni, N. (2006) Mapping regulatory genes as candidates for cold and drought stress tolerance in barley. Theor. Appl. Genet. 112:445-454. http://www.ncbi.nlm.nih.gov/pubmed/16315028

Skinner, J.S., Szűcs, P., von Zitzewitz, J., Marquez-Cedillo, L., Filichkin, T., Stockinger, E.J., Chen, T.H.H., and Hayes P.M. (2006) Mapping of barley homologs to genes that regulate low temperature tolerance in Arabidopsis. Theor. Appl. Genet. 112:832-842. http://www.ncbi.nlm.nih.gov/pubmed/16365758

Skinner, J.S., von Zitzewitz, J., Szűcs, P., Marquez-Cedillo, L., Filichkin, T., Amundsen, K., Stockinger, E.J., Thomashow, M.F., Chen, T.H.H., and Hayes P.M. (2005) Structural, functional and phylogenetic characterization of a large CBF gene family in barley. Plant Mol. Biol. 59:533-551. http://www.ncbi.nlm.nih.gov/pubmed/16244905

Wang, Z., Triezenberg, S.J., Thomashow, M.F., and Stockinger E.J. (2005) Multiple hydrophobic motifs in Arabidopsis CBF1 COOH-terminus provide functional redundancy in trans-activation. Plant Mol. Biol. 58:543-559. http://www.ncbi.nlm.nih.gov/pubmed/16021338

Zhang, X., Fowler, S.G., Cheng, H., Lou, Y., Rhee, S.Y., Stockinger, E.J., and Thomashow, M.F. (2004) Freezing sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing tolerant Arabidopsis. Plant J. 39:905-919. http://www.ncbi.nlm.nih.gov/pubmed/15341633

Coaker, G.L., Willard, B., Kinter, M., Stockinger, E.J., and Francis, D.M. (2004) Proteomic analysis of resistance mediated by Rcm 2.0 and Rcm 5.1, two loci controlling resistance to bacterial canker of tomato. Mol. Plant Microbe Interact. 17:1019-1028. http://www.ncbi.nlm.nih.gov/pubmed/15384492

Stockinger, E.J., Mao, Y., Regier, M., Triezenberg, S.J. and Thomashow, M.F. (2001) Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Nucleic Acids Res. 29:1524-1533. http://www.ncbi.nlm.nih.gov/pubmed/11266554

Thomashow, M.F., Gilmour, S.J., Stockinger, E.J., Jaglo-Ottosen, K.R., Zarka, D.G. (2001) Role of the Arabidopsis CBF transcriptional activators in cold acclimation. Physiol. Plant. 112:171-175.

Gilmour, S.J., Zarka, D.G., Stockinger, E.J., Salazar, M.P., Houghton, J.M., and Thomashow, M.F. (1998) Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J. 16:433-442. http://www.ncbi.nlm.nih.gov/pubmed/9881163

Stockinger, E.J., Gilmour, S.J. and Thomashow, M.F. (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad. Sci. U.S.A. 94:1035-1040. http://www.ncbi.nlm.nih.gov/pubmed/9023378

Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., Gilmour, S.J. and Zarka, D.G. (1997) Function and regulation of Arabidopsis thaliana COR (cold-regulated) genes. ACTA Physiol. Planta. 19:497-504. http://www.springerlink.com/content/8528824533846tu4/

Garvin, D.F., Federici, C.V., Stockinger, E.J., and Waines, J.G. (1997) Genetic marker transmission in early generation common x tepary bean hybrids. J. Hered. 88:537-540. http://jhered.oxfordjournals.org/content/88/6/537

Stockinger, E.J., Mulinix, C.A., Long, C.M., Brettin, T.S. and Iezzoni, A.F. (1996) A linkage map of sweet cherry based on RAPD analysis of a microspore-derived callus culture population. J. Hered. 87:214-218. http://www.ncbi.nlm.nih.gov/pubmed/8683097

Stockinger, E.J., and Walling, L.L. (1994) Pto3 and Pto4: novel genes from Lycopersicon hirsutum var. glabratum conferring resistance to Pseudomonas syringae pv. tomato. Theor. Appl. Genet. 89:879-884. http://www.springerlink.com/content/l64qx21741w46496/

Stockinger, E.J., and Walling, L.L. (1994) A chlorophyll a/b-binding protein gene from soybean (Glycine max. [L.] Merr). Plant Physiol. 104:1475-1476. http://www.ncbi.nlm.nih.gov/pubmed/8016279

Demmin, D.S., Stockinger, E.J., Chang, Y.C., and Walling, L.L. (1989) Phylogenetic relationships between chlorophyll a/b-binding protein (CAB) multigene family: An intra - and interspecies study. J. Mol. Evol. 29:266-279. http://www.ncbi.nlm.nih.gov/pubmed/2506360

 

Book Chapters

Galiba, G., Stockinger, E.J., Francia, E., Milc, J., Kocsy, G., and Pecchioni, N. (2013). Freezing tolerance in the Triticeae. In Translational Genomics for Crop Breeding, R.K. Varshney, and R. Tuberosa, eds. (USA: Wiley), pp. 99–124.

Stockinger, E.J. (2011). Pipkin. In, “The Oxford Companion to Beer,” G. Oliver (ed.) Oxford University Press, New York. pp. 656.

Stockinger EJ (2009) Winter hardiness and the CBF genes in the Triticeae. In, “Plant Cold Hardiness From the Laboratory to the Field,” LV Gusta, M Wisniewski and KK Tanino, (eds.), CABI Publishing Oxon, UK. pp. 119-130.

Stockinger, E.J., Cheng, H., and Skinner, J.S. (2005) Structural organization of barley CBF genes coincident with a QTL for cold hardiness. In, “Cold Hardiness in Plants,” THH Chen, M Uemura and S Fujikawa, (eds.), CABI Publishing, Wallingford, Oxon, UK. pp. 53-63.

Skinner, J.S., von Zitzewitz, J., Marquez-Cedillo, L., Filichkin, T., Szûcs, P., Amundsen, K., Stockinger E.J., Thomashow, M.F., Chen, T.H.H., and Hayes, P.M. (2006) Barley Contains a Large CBF Gene Family Associated with Quantitative Cold Tolerance Traits. In, “Cold Hardiness in Plants,” THH Chen, M Uemura and S Fujikawa, (eds.), CABI Publishing Oxon, UK pp. 30-52.

Thomashow, M.F., Stockinger, E.J., and Gilmour, S.J. (1997) Regulation of plant gene expression in response to low temperature. In, “Plant Cold Hardiness. Molecular Biology, Biochemistry and Physiology,” PH Li and THH Chen (eds.), Plenum, New York. pp. 29-34.

Thomashow, M.F., Artus, N.N., Bloksberg, L.N., Gilmour, S.J., Stockinger, E.J., Wilhelm, K., Uemura, M., Webb, M.S., and Steponkus, P.L. (1996) Cold acclimation in Arabidopsis thaliana: function and regulation of COR genes. In, “Physical Stresses in Plants. Genes and their Products for Tolerance,” S. Grillo and A. Leone (eds.) Springer, Berlin. pp. 71-81.

 

Patents Awarded

Thomashow, M.F., Gilmour, S.J., Jiang, C-Z., Fromm, M., Zarka, D.G., Jaglo-Ottosen, K.R., Stockinger, E.J., Zhang, J., and Haake, V. (2004) Plant having altered environmental stress tolerance. Patent no. 6,706,866

Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., Gilmour, S.J., Zarka, D.G., and Jiang, C-Z. (2002) Plant having altered environmental stress tolerance. Patent no. 6,417,428

Thomashow, M.F., and Stockinger, E.J. (1999) DNA and encoded protein which regulates cold and dehydration regulated genes. Patent no. 5,965,705

Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., and Zarka, D.G. (1999) Plant material containing non-naturally introduced binding protein for regulating cold and dehydration regulatory genes. Patent no. 5,929,305

Thomashow, M.F., and Stockinger, E.J. (1999) DNA and encoded protein which regulates cold and dehydration regulated genes. Patent no. 5,892,009

Thomashow, M.F., Stockinger, E.J., Jaglo-Ottosen, K.R., Zarka, D.G., and Gilmour, S.J. (1999) Method for regulating cold and dehydration regulatory genes in a plant. Patent no. 5,891,859